Nucleotide sequence of Escherichia coli K-12 replication origin.
نویسندگان
چکیده
منابع مشابه
Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome.
The origin of replication, oriC, of the Escherichia coli chromosome was mapped within a DNA segment of 422 base pairs. The nucleotide sequence of this segment was determined. The source of DNA for the sequence analysis was a minichromosome constructed in vivo, consisting exclusively of chromosomal DNA and a minichromosome constructed by cloning in vitro. The nucleotide sequence of the replicati...
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The tryptophanase structural gene, tnaA, of Escherichia coli K-12 was cloned and sequenced. The size, amino acid composition, and sequence of the protein predicted from the nucleotide sequence agree with protein structure data previously acquired by others for the tryptophanase of E. coli B. Physiological data indicated that the region controlling expression of tnaA was present in the cloned se...
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The xth gene of Escherichia coli K-12, which encodes exonuclease III, has been sequenced. Exonuclease III from a cloned copy of the E. coli K-12 gene has been purified and characterized. The molecular weight (30,921), the amino-terminal amino acid sequence, and the amino acid composition of the polypeptide predicted from the nucleotide sequence are in excellent agreement with those properties d...
متن کاملNucleotide sequence of the lexA gene of Escherichia coli K-12.
A number of E. coli genes exhibit increased expression when the cellular DNA is damaged. In undamaged cells, lexA repressor limits the extent of their transcription, whereas, in damaged cells, the repressor is cleaved by a cellular protease, the product of the recA gene. We have sequenced 943 base pairs of cloned E. coli DNA containing the lexA gene. A regulatory region has been identified, fol...
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The nfo gene of Escherichia coli K-12 which encodes endonuclease IV has been sequenced. The predicted gene product has a molecular weight of 31,562, in good agreement with the size of the gene product estimated by maxicell analysis. The nfo promoter was mapped by primer extension of in vivo transcripts. Inspection of the nucleotide sequence revealed no regions of potential secondary structure c...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1979
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.76.2.575